adrenocortical carcinoma cell line sw13  (ATCC)

Journal: Nature communications

Article Title: A PRPH splice-donor variant associates with reduced sural nerve amplitude and risk of peripheral neuropathy.

doi: 10.1038/s41467-019-09719-4

Figure Lengend Snippet: Fig. 5 Truncated PRPH variants generate protein inclusions. SW13 cells were transfected with a wild-type (PRPH WT) using n = 332 cells, b 4bp-splice variant (PRPH 4 bp) using n = 545 cells, or c intron retention splice-variant (PRPH Intron), cultured for 48 h and labeled with an antibody raised against peripherin and counterstained with DAPI (4′,6-diamidino-2-phenylindole) for nuclear visualization, using n = 177 cells. Scale bar represents 10 µm. d Peripherin filaments and inclusions were quantified from the three independent experiments (n = 177–545). Inclusions were subdivided by size; fibrous inclusions ≥5 µm, large inclusions 1.5–5 µm, and small inclusions ≤1.5 µm (n = 177–545). Error bars represent standard deviations (SD)

Article Snippet: The SW13 adrenocortical carcinoma cell line was purchased from ATCC (CCL-105) and cultured at 37 °C, 5% CO2 in Dulbecco’s modified Eagle's medium (31966-021; Gibco) supplemented with glutamax, 10% fetal bovine serum (10500-064; Gibco), 100 Uml−1 penicillin and 100 μg ml−1 streptomycin (15140-122; Gibco).

Techniques: Transfection, Variant Assay, Cell Culture, Labeling

Journal: Oncotarget

Article Title: Overexpression of HSD17B4 exerts tumor suppressive function in adrenocortical carcinoma and is not associated with hormone excess

doi: 10.18632/oncotarget.22827

Figure Lengend Snippet: ( A ) constitutive HSD17B4 level; ( B ) robust knockdown (KD) of HSD17B4 using shRNAs in both cell lines and ( C ) robust upregulation of HSD17B4 in both cell lines; HSD17B4-KD and overexpression in NCI-H295 and SW13 cells effecting on ( D ) proliferation, ( E ) cell cycle and ( F ) cell apoptosis, respectively ( ** P < 0.01, n = 3).

Article Snippet: The human adrenocortical cancer (NCI-H295R) and adrenocortical small cell carcinoma (SW13) cell lines were originally obtained from ATCC and cultured in DMEM medium (Thermo Scientific, Logan, UT, USA) supplemented with 20% fetal bovine serum.

Techniques: Knockdown, Over Expression

Journal: Oncotarget

Article Title: Overexpression of HSD17B4 exerts tumor suppressive function in adrenocortical carcinoma and is not associated with hormone excess

doi: 10.18632/oncotarget.22827

Figure Lengend Snippet: ( A ) reproduction of TCGA ACC dataset showing heatmap of p53 signaling related genes in cases with and without HSD17B4 overexpression; ( B ) MDM4 expression is significantly lower in cases with HSD17B4 overexpression; ( C ) ATR expression is significantly higher in cases with HSD17B4 overexpression; enriched genes were confirmed for protein level using western blotting in ( D ) H295R cells with and without HSD17B4-KD, and in ( E ) SW13 cells with and without HSD17B4 replenish ( * P < 0.05).

Article Snippet: The human adrenocortical cancer (NCI-H295R) and adrenocortical small cell carcinoma (SW13) cell lines were originally obtained from ATCC and cultured in DMEM medium (Thermo Scientific, Logan, UT, USA) supplemented with 20% fetal bovine serum.

Techniques: Over Expression, Expressing, Western Blot

Journal: Oncotarget

Article Title: Overexpression of HSD17B4 exerts tumor suppressive function in adrenocortical carcinoma and is not associated with hormone excess

doi: 10.18632/oncotarget.22827

Figure Lengend Snippet: HSD17B4-KD and overexpression in NCI-H295 and SW13 cells effecting on ( A ) cell invasion; ( B ) cell migration; and ( C ) colony formation, respectively; ( D ) HSD17B4-KD induced increased in vivo tumor growth of NCI-H295R cells ( ** P < 0.01, NS = not significant, n = 3).

Article Snippet: The human adrenocortical cancer (NCI-H295R) and adrenocortical small cell carcinoma (SW13) cell lines were originally obtained from ATCC and cultured in DMEM medium (Thermo Scientific, Logan, UT, USA) supplemented with 20% fetal bovine serum.

Techniques: Over Expression, Migration, In Vivo